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Post by whiterose on Mar 26, 2007 17:24:27 GMT -5
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Post by whiterose on Aug 29, 2007 7:58:43 GMT -5
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Post by maggiemae on Aug 29, 2007 21:30:13 GMT -5
Well, funny you should mention this....ok, here we go again...member the last time and I wigged out really bad here? I checked out a name on my call id again...has been listed twice in a week. It said NSI Group...so I did a google on it....this site popped up. Hummmm...so I clicked on a link and got this.....are we being given crumbs? www.nano.ecs.soton.ac.uk/Research/Bioelectronics_and_Lab_on_a_chipnote the bionanotechnology part? This is interesting this time, not scary like last time... I mean I know there are other acronyms but again, what are the odds..... Mm Anybody have this show up on your id? Just turnen' stones..
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Post by maggiemae on Aug 29, 2007 21:49:29 GMT -5
Changes in a neutralization domain of sequential isolates in experimental and natural HIV infection.
Kuiken CL, DeJong JJ, Tersmette M, Smit L, Nara PL, Goudsmit J.
Int Conf AIDS. 1990 Jun 20-23; 6: 151 (abstract no. S.A.278). Human Retrovirus Laboratory, Academic Medical Center, Amsterdam, the Netherlands
OBJECTIVE: To study changes in the coding region (gp120 V3) for a neutralization epitope of non-syncytium-inducing (NSI) and syncytium inducing (SI) isolates. METHODS: Sequential samples from 2 men were studied in whom HIV-1 virus changed from NSI to SI virus. From these, DNA was obtained both before and after co-cultivation. Two chimpanzees were infected with an inoculum grown from an SI stock (IIIB) consisting of HXB2, HXB3 and HX10 infectious clones. This stock was studied after passage in both human and chimpanzee cells. In all cases, the V3 domain (approx. 105 nucleotides) was sequenced. RESULTS: Sequences from cocultivated material of 7 samples from 1 man showed a distinct change from the NSI (sample 1 of 4, yearly rate 2.5% change) to the SI virus (samples 5 to 7, yearly rate of 0.7%). Between samples 4 and 5 the phenotype shifted (yearly rate 23%). The 3 samples available from the other man showed a comparable change. When clustered, the SI and NSI sequences were distinct from each other. Sequences before and after co-cultivation were similar in 3 out of 4 cases studied. Chimpanzee V3 sequences from 6, 16 and 32 weeks post infection, diverged from the inoculum by 3 to 4 nucleotides of 108, parallel to escaping V3 specific antibodies, and 0-2 nucleotides from each other. Cluster analysis lumped the inoculum with the in vitro passaged sequences and HXB3, possibly reflecting speed of growth, whereas the in vivo passaged sequences resembled HX10, HXB2 and the IIIB stock. CONCLUSION: The V3 domain of SI virus does not change faster than that of NSI virus. Transition from NSI to SI is reflected in an abrupt change in the V3 domain. Genomes analyzed prior to culture show the same pattern as those analyzed after culture. In experimental infection the highly passaged inoculum itself resembled sequences resulting from in vitro passage of the IIIB stock. Sequences resulting from the in vivo experiment became similar to the stock from which the inoculum was grown.
Skytroll....this one is up your alley I would say....I typed in NSI experimental...this popped up....see God works in mysterious ways, so I thought I'd play around a bit with the letters, NSI...one thing that drew me in was the location....whether this is relevant or just a goose, I got a really strong gut feeling Netherlands are involved...I think T2 mentioned them frequently...yes?
Whiterose, I do apologize....I meant to put this on a different thread...so sorry...., Mm
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