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Post by whiterose on Nov 6, 2006 17:41:53 GMT -5
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Post by mankind on Nov 6, 2006 19:46:17 GMT -5
Can I call this placebo effect of marketing?
One thing is sure, these peoples who buy anything and think it work cause they see it on TV or the box. Credibility is added when everyone use it, true? Plus the company must be trustable they are rich anyway. Even if its true sometime whats the cost you paid after...!?
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Post by whiterose on Nov 6, 2006 20:48:00 GMT -5
So true, no one thinks about after, they only concern themselves with now. Do they not realize that after is our children and their children.
whiterose
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Post by gracebeours on Dec 16, 2006 8:32:26 GMT -5
Note the references to gold, positive and negative charges and PH levels. (a) Can I link more than one molecule to Nanogold®?
Positively Charged Nanogold® and Negatively Charged Nanogold® both contain multiple functional groups (amines and carboxyls respectively) that may be activated for cross-linking. Therefore, it is feasible to link more than one entity to them. However, while this is an effective strategy for linking several copies of the same entity, we do not recommend it for successive conjugation reactions. Only one type of reactivity is incorporated into Nanogold. This makes it extremely difficult to control the reaction stoichiometry to ensure that the first conjugation yields predominantly the desired conjugate, while sufficient unreacted functionalities remain for the second reaction. In addition, if you have activated the gold, it will be very difficult to remove either gold that has failed to react in the first step, or unconjugated biological molecule, before the activated group is hydrolyzed.
In many cases, you may be able to link the three components together in a different conformation much more easily. If one is a larger molecule that has more than one reactive functional group, then this will be the one that can support multiple attachments, and you can label it sequentially at two different sites using different cross-linking chemistries. This is how FluoroNanogold is made: first Monomaleimido-Nanogold is linked to a hinge thiol, then an amine-reactive fluorescent label is conjugated to an amine elsewhere in the Fab' fragment.
For example, if you wish to link both Nanogold and an oligonucleotide to an antibody, the best strategy is to first label the IgG with Monomaleimido-Nanogold, to attach the gold selectively to the hinge region. This positions the gold away from the antigen combining region, and leaves the amino- groups elsewhere on the molecule intact for the second step. Then, once you have purified the Nanogold-IgG conjugate, activate the DNA for cross-linking to amines - for example, incorporate a 5'-amino modifier during synthesis and activate with a heterobifunctional cross-linker such as bis-(sulfo-scuccinimidyl)-suberate, or BS3. React this with the IgG. Of course, if you decide it makes more sense to position the oligo in the hinge region, you can do this the other way round - activate the oligonucleotide for thiol reactivity, couple to the hinge region of the reduced IgG, then label the conjugate with Mono-Sulfo-NHS-Nanogold (remember not to use thiolated reagents after Nanogold labeling, as these can displace or degrade the gold).
(b) Does Nanogold® or undecagold ionize?
Many of you wish to use charge-based separation methods, such as gel electrophoresis or ion exchange chromatography, to isolate gold conjugates, and are interested to know what charge the Nanogold® and undecagold reagents have.
Both Nanogold and undecagold are molecular coordination compounds. The gold core itself does not have any residual charge, but is fully stabilized and capped by coordinated ligands. The only charge that these molecules have is that of chemical groups that we incorporate into the coordinated ligands to impart reactivity or functionality. For example, Positively Charged Nanogold is protonated at intermediate to low pH values; Negatively charged Nanogold is deprotonated at mid to high pH values. However, when Monomaleimido- and Mono-Sulfo-NHS-Nanogold react as intended, they form respectively a thioether or an amide cross-link. Neither of these may be protonated or deprotonated under the conditions in which gold conjugates are stable.
This means that labeling with Monomaleimido Nanogold will not affect the charge of the conjugate biomolecule, and therefore the only change it will impart in charge-based separation procedures is that of the added mass of the gold (although other factors may affect gel electrophoresis separation). Labeling with Mono-Sulfo-NHS-Nanogold will remove one amine from the conjugate biomolecule, and its ionization behavior will be modified accordingly.
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Post by aussie on Dec 16, 2006 21:16:58 GMT -5
Hi there Gracebeours,
Thank you for the technical information......now can somebody tell me in layman's terms what it all means??
I imagine you have a reference?
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Post by whiterose on Dec 21, 2006 0:45:56 GMT -5
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Post by aligator on Jan 2, 2007 17:03:02 GMT -5
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Post by aligator on Jan 2, 2007 17:48:39 GMT -5
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Post by whiterose on Jan 5, 2007 16:29:41 GMT -5
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Post by whiterose on Jan 12, 2007 11:00:28 GMT -5
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Post by whiterose on Jan 16, 2007 16:20:05 GMT -5
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Post by whiterose on Jan 18, 2007 12:55:49 GMT -5
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Post by whiterose on Jan 18, 2007 23:38:20 GMT -5
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Post by whiterose on Jan 25, 2007 21:49:37 GMT -5
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Post by aligator on Jan 25, 2007 23:43:07 GMT -5
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Post by whiterose on Jan 26, 2007 3:39:37 GMT -5
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Post by whiterose on Jan 27, 2007 19:27:56 GMT -5
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Post by whiterose on Feb 6, 2007 15:25:33 GMT -5
Personally, I wonder if this is not a post dated article. We see so many things that are to come, but if you look really close, you see they already are. The crowd disperser is one example, where it said it was on the drawing board, when in fact it was already being used, interesting don't you think? This your wakeup call, it is the 7th one! (Thank you gazal) www.raidersnewsnetwork.com/full.php?news=2573
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Post by whiterose on Mar 7, 2007 18:05:18 GMT -5
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Post by whiterose on Mar 8, 2007 5:32:43 GMT -5
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Post by sarahconnor on Mar 8, 2007 16:50:38 GMT -5
"The potential of this new science to create artificially "enhanced humans" who are supposed to be smarter, stronger and have other capabilities such as night vision."
Now the thought of the night vision wouldn't be a bad idea, especially with all those trips to the loo in the night!!
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Post by whiterose on Mar 17, 2007 8:07:11 GMT -5
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Post by whiterose on Mar 17, 2007 11:35:07 GMT -5
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Post by whiterose on Mar 20, 2007 17:38:36 GMT -5
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Post by whiterose on Mar 21, 2007 20:25:40 GMT -5
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Post by whiterose on Mar 21, 2007 20:52:01 GMT -5
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