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Post by whiterose on Sept 18, 2007 11:41:05 GMT -5
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Post by skytroll on Sept 18, 2007 13:36:46 GMT -5
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Post by skytroll on Sept 18, 2007 13:42:31 GMT -5
Let's hit them where it hurts>..... COLL 6 One-pot encapsulation of enzymes and nanoparticles using silica precipitating peptides Melanie M. Tomczak, Maneesh K. Gupta, and Rajesh R. Naik, Materials and Manufacturing Directorate, MLPJ Biotechnology Group, Air Force Research Laboratory, 3005 Hobson Way, Wright-Patterson Air Force Base, Dayton, OH 45433, rajesh.naik@wpafb.af.mil The successful use of enzymes for applications in catalysis and sensors is dependent on the host material used for immobilization of the enzymes. One of the most widely used method for immobilizing enzymes is sol-gel silica encapsulation. Here we describe an alternative method that mimics the diatom biosilicification process for the entrapment of enzymes in a silica matrix using silica precipitating peptides. The entrapment process is a one-pot procedure wherein the silica matrix is synthesized using a peptide, in the presence of the enzyme to be immobilized. Larger macromolecules and nanoparticles were also found to be encapsulated using this approach. The encapsulated enzymes were found to be stable and also exhibited heat stability compared to the free enzyme. -------------------------------------------------------------------------------- Just what our Dr. was saying.................... encapsulation inside cells....................................... tinyurl.com/3blt8mhappy kicking.......................... s
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Post by skytroll on Sept 18, 2007 13:43:43 GMT -5
HELLO>>> Two silica precipitating peptides, silaffin-1A1 and -1A2, both encoded by the sil 1 gene from the diatom Cylindrotheca fusiformis, were extracted from cell walls and purified to homogeneity. The chemical structures were determined by protein chemical methods combined with mass spectrometry. Silaffin-1A1 and –1A2 consist of 15 and 18 amino acid residues, respectively. Each peptide contains a total of four lysine residues, which are all found to be posttranslationally modified. In silaffin-1A2 the lysine residues are clustered in two pairs in which the -amino group of the first residue is linked to a linear polyamine consisting of 5 to 11 N-methylated propylamine units, whereas the second lysine is converted to -N,N-dimethyl-lysine. Silaffin-1A1 contains only a single lysine pair exhibiting the same structural features. One of the two remaining lysine residues was identified as -N,N,N-trimethyl--hydroxylysine, a lysine derivative containing a quarternary ammonium group. The fourth lysine residue again is linked to a long-chain polyamine. Silaffin-1A1 is the first peptide shown to contain -N,N,N-trimethyl--hydroxylysine. In vitro, both peptides precipitate silica nanospheres within seconds when added to a monosilicic acid solution. www.jbc.org/cgi/content/abstract/M102093200v1www.pubmedcentral.nih.gov/articlerender.fcgi?artid=18883help me people................... hot...........diggity! s
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Post by skytroll on Sept 18, 2007 13:44:49 GMT -5
Silica from diatoms...............................yes.................j
come on back you who know.................................................
s
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Post by skytroll on Sept 18, 2007 13:48:42 GMT -5
cylindrotheca fusiformis
the way in......................but it is a nano...........................
can be bio and chemo and physico........
s
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Post by skytroll on Sept 18, 2007 13:49:59 GMT -5
It is molecular electronics.........the Electronic spore..........
It has been done wrong............................
s
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Post by skytroll on Sept 18, 2007 13:53:10 GMT -5
can you all see it? Theoretical descriptions of electrical transport through molecular wires show that contacts, coupling of contacts to molecules, interface geometries, and vibrations are important in single-molecule experiments (16–21). For studies on a large ensemble of molecules some of these effects will average out over all of the molecules. Consequently, differences in electrical transport between measurements on single molecules and self-assembled monolayers (SAMs) were observed experimentally (ref. 5, ref. 22 and references therein, and ref. 23). Alkane(di)thiols are nonconducting molecules with a bandgap of 8–10 eV (ref. 10 and ref. 22 and references therein) and consist of a saturated carbon backbone with one (or two) thiol end groups. Experimentally, the tunneling current through a monolayer of alkane(di)thiols was shown to be temperature-independent and to decrease exponentially with increasing molecular length (4, 5). The transport has been interpreted in terms of the classical tunneling model through a thin insulating film as provided by Simmons (24, 25). In this model the tunneling current depends on the mean value of the barrier height, allowing for a simplification of the problem of an arbitrarily shaped potential barrier to that of a rectangular barrier. This model has been applied to junctions based on SAMs (4, 26), but an extra fit parameter is needed to obtain a fit to the measured data. However, as already explained by Simmons (24, 25), for a practical tunnel junction the image potential has to be taken into account. This effect has been neglected in the literature so far. www.pnas.org/cgi/content/full/104/27/11161s
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Post by skytroll on Sept 18, 2007 22:06:54 GMT -5
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Post by skytroll on Sept 18, 2007 22:09:08 GMT -5
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Post by skytroll on Sept 18, 2007 22:10:41 GMT -5
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praying4usall
Full Member
He shall cover you with His Feathers, under His Wings you will find Refuge.
Posts: 244
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Post by praying4usall on Sept 18, 2007 22:14:22 GMT -5
Xintek Received New Phase II SBIR Contract from the U.S. Air Force Research Triangle Park, NC (February 1, 2006). Xintek has received a new 2-year Phase II SBIR contract from the U.S. Air Force to develop field emission based high-power microwave tubes for defense related applications. The technology is based on Xintek’s proprietary nanomaterial based field emission technologies. The project includes a sub-contract to a major microwave tube manufacturer.
Xintek Awarded New Government Contract from NIH Research Triangle Park, NC (July 15, 2005). Xintek announced that it has been awarded a new government contract from the National Institute of Health (NIH). This contracted work focuses on the development of a new field emission x-ray source for advanced biomedical imaging. The device will be based on Xintek’s patented carbon nanotube field emission x-ray technologies.
Xintek Inc. Nanotechnology Innovations
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Post by skytroll on Sept 18, 2007 22:15:59 GMT -5
Okay, let's get to it?> jxb.oxfordjournals.org/cgi/content/abstract/17/3/473Physiological Studies of the Boron Requirement of the Diatom, Cylindrotheca fusiformis Reimann and Lewin JOYCE C. LEWIN Scripps Institution of Oceanography, University of California La Folla, Calif., U.S.A. Cylindrotheca fusiformis, a marine pennate diatom, requires boron for growth both in the light and in the dark. A synthetic saline nutrient medium and plastic containers were used to demonstrate the boron requirement. A boron concentration of at least 0.5 ppm supports the maximum growth-rate, with a generation time of approximately 10 hours. Boron concentrations below 0.5 ppm result in growth at a reduced rate, the effect being most apparent during the first few days of growth of the culture. The growth-rate is also affected by the initial silicon: boron ratio in the medium, indicating some possible interaction between these two elements. Boron necessary is in the chemtrails.....................
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praying4usall
Full Member
He shall cover you with His Feathers, under His Wings you will find Refuge.
Posts: 244
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Post by praying4usall on Sept 18, 2007 22:18:46 GMT -5
Chemists create, grow nanotube seeds Rice University chemists today revealed the first method for cutting carbon nanotubes into "seeds" and using those seeds to sprout new nanotubes. The findings offer hope that seeded growth may one day produce the large quantities of pure nanotubes needed for dozens of materials applications. www.physorg.com/news82986478.html
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praying4usall
Full Member
He shall cover you with His Feathers, under His Wings you will find Refuge.
Posts: 244
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Post by praying4usall on Sept 18, 2007 22:26:26 GMT -5
Actually, my Brother just sent me these links, as he is now knowing I'm OK mentally, (lol! Well, sorta)! and that there IS something WRONG going on! I am so glad! Both he and his wife are have been working with the Dept of Ecology for about 20 years now. Thank you Lord! It is good to be believed when you really love your family, and at first my kids were quite concerned, since the so called Drs who I refused to go to anymore didn't find anything wrong...( yet...they never DID wear GLOVES when they ever bothered to actaully TOUCH me)! I would never wish this on them though!! Especially my really NICE DR.!
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Post by skytroll on Sept 18, 2007 22:51:53 GMT -5
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Post by skytroll on Sept 18, 2007 22:52:46 GMT -5
praying4usall,
there is evidence that it is in the chemtrails.............
s
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Post by skytroll on Sept 18, 2007 22:55:14 GMT -5
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Post by skytroll on Sept 18, 2007 23:02:42 GMT -5
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praying4usall
Full Member
He shall cover you with His Feathers, under His Wings you will find Refuge.
Posts: 244
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Post by praying4usall on Sept 18, 2007 23:02:47 GMT -5
Yup!
I'm sure we have only seen a tiny bit of just the tip of the iceberg! I am glad we have the Net to 'net' with!
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praying4usall
Full Member
He shall cover you with His Feathers, under His Wings you will find Refuge.
Posts: 244
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Post by praying4usall on Sept 18, 2007 23:10:07 GMT -5
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praying4usall
Full Member
He shall cover you with His Feathers, under His Wings you will find Refuge.
Posts: 244
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Post by praying4usall on Sept 18, 2007 23:12:23 GMT -5
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Post by skytroll on Sept 18, 2007 23:17:45 GMT -5
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Post by skytroll on Sept 18, 2007 23:20:07 GMT -5
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Post by skytroll on Sept 18, 2007 23:25:13 GMT -5
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Post by skytroll on Sept 18, 2007 23:26:01 GMT -5
praying4usall,
thank you so very much. you have helped so much...
love ya, and see you on the morrow.
skytroll
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Post by whiterose on Oct 10, 2007 17:32:19 GMT -5
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Post by whiterose on Oct 10, 2007 22:07:33 GMT -5
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Post by whiterose on Oct 15, 2007 9:56:36 GMT -5
cnews.canoe.ca/CNEWS/WeirdNews/2007/10/10/4565372-ap.htmlPhantom Vibrations Research in the area is scant, but theories abound about the phenomenon, which has been termed "ringxiety" or "fauxcellarm." Anecdotal evidence suggests "people feel the phone is part of them" and "they're not whole" without their phones, since the phones connect them to the world, said B.J. Fogg, director of research and design at Stanford University's Persuasive Technology Lab. "As human beings, we're so tapped into our community, responsiveness to what's going on, we're so attuned to the threat of isolation and rejection, we'd rather make a mistake than miss a call," he said. "Our brain is going to be scanning and scanning and scanning to see if we have to respond socially to someone." In certain circles, phantom vibrations are a point of pride. Thank Your Raiders News Network!
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